Adsorption of Blood Creatinine on a Heparin‐Functionalized Surface of Copper‐Based Metal–Organic Frameworks

ABSTRACT Introduction Creatinine accumulation exacerbates outcomes in chronic kidney disease (CKD). To enhance creatinine removal and improve hemocompatibility, we developed heparin‐functionalized copper‐based metal–organic frameworks (CuMOFs‐Hep). Methods CuMOFs were heparinized via APTES bridging and characterized (XRD, SEM, FTIR, XPS, and zeta potential). Adsorption was evaluated in simulated CKD serum and was fitted with isotherm models. Whole‐blood samples from patients/healthy donors were tested. Biosafety was assessed by Cu 2+ leaching and platelet assays. Results Heparinization increased surface negative charge and raised the maximum capacity to 267.8 mg g −1 (68.7% over pristine; Langmuir R 2 = 0.983). Adsorption kinetics studies indicate rapid clearance of creatinine, achieving a clearance rate of 65.9% within 30 min at 37°C and reaching 75.0% within 2 h. In patient blood (854.2 μmol/L), supernatant creatinine decreased to 163.2 μmol/L (80.9% removal). Cu 2+ leaching dropped to 0.214 ppm (−89.9%), with reduced platelet adhesion/activation. Conclusion CuMOFs‐Hep enables efficient, low‐leaching creatinine adsorption in clinically relevant matrices, highlighting its promising potential as an efficient adsorbent for blood purification applications.