Combining Electrochemical Reduction with Biosynthesis for Directed Conversion of CO 2 into a Library of C3 Chemicals

ABSTRACT Electrochemical carbon dioxide (CO 2 ) reduction presents significant opportunities for sustainable chemical manufacturing. However, conventional electrochemical CO 2 reduction typically produces only C1 or C2 products, and the direct synthesis of C3 chemicals remains a major challenge. In this study, we developed a unique tandem system integrating microbial electroreduction with biosynthesis, demonstrating the feasibility of using CO 2 ‐derived acetic acid as a carbon source for biosynthesis. In Module I, taking advantage of an established perfluorocarbon nanoemulsion strategy for enhanced H 2 delivery, we achieved efficient electrocatalytic CO 2 ‐to‐acetate conversion with an acetic acid production rate of 0.34 ± 0.01 g L −1 day −1 . In Module II, the engineered was established by developing an ultrahigh mutation system to facilitate the screening of desirable microbes with high toxicity tolerance to acetic acid and different products. Various C3 chemicals were efficiently synthesized by this tandem system, including β‐alanine (2.1 g/L), acrylic acid (752.4 mg/L), and L‐lactic acid (672.9 mg/L), achieving a conversion yield of 0.54–0.72 tons of destined products per ton of CO 2 with an energy consumption of only 51.88–80.7 GJ. This study introduced a novel approach for upcycling CO 2 into a library of high‐value C3 chemicals, offering a new approach for achieving carbon neutrality.