Deoxysugar Formation via 4',5'‐Dehydration on PKS Assembly Line in Nucleoside Antibiotic Biosynthesis

Angewandte Chemie International Edition 2026

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Authors: Zi‐Hui Chen, Fusong Wang, Weiyan Wang, Tian‐Ci Zhang, Yuxuan Wang, Wen‐He Zhang, Jinyue Pu, Aiai Sun, Hai‐Xue Pan, Gong‐Li Tang
DOI: 10.1002/anie.6927983
Cited by: 1
References: 26

Abstract

high-carbon sugar, with potent activity against the rice blast pathogen Pyricularia oryzae. The biosynthetic machinery of the C-4' methylene group in its deoxy sugar has remained unknown. This study reveals that ApmL, a hypothetical protein in the DUF3500 family, functions as a 4',5'-dehydratase whose activity is strictly dependent on the PKS assembly line. Together with its partner reductase ApmM, they complete a two-step C-4' deoxygenation process via dehydration-reduction. We achieve the first in vitro reconstitution of this unique PKS-coupled dehydration process and show direct interaction between ApmL and PKS proteins. Our findings establish ApmL as a new family of dehydratase and uncover a noncanonical deoxygenation strategy in nucleoside antibiotic biosynthesis.

Fields of Study & Subjects

Biochemical and Molecular Research Enzyme Structure and Function Peptidase Inhibition and Analysis

Publication details

Journal / source
Angewandte Chemie International Edition
Publisher
Wiley
Volume
65
Issue
19
Pages
e6927983–e6927983
Publication date
28 March 2026
Language
EN