Co‐Infection of Brucella canis and Brucella melitensis in a Domestic Dog With Zoonotic Transmission to Human, China

ABSTRACT We report a rare occurrence of genetic material from both Brucella canis and B. melitensis detected in a domesticated dog from Jiangsu Province, China. The detection was confirmed through serological assays, quantitative PCR (qPCR), metagenomic and genomic sequencing. Epidemiological evidence and whole genome sequencing suggested zoonotic transmission from the dog to its owner, who was diagnosed with brucellosis. Although isolation of both Brucella species was unsuccessful, the presence of dual genetic material highlights diagnostic complexities and underscores the zoonotic risks posed by companion animals, necessitating enhanced diagnostic awareness and preventive measures.