Enhancing DNAzyme Silencing via Phosphorothiolation to Reduce the Mg 2+ ‐Dependence
Abstract
ABSTRACT 817 DNAzyme has emerged as a potent catalytic nucleic acid tool for gene expression silencing, offering distinct advantages including programmable target recognition, enzymatic turnover capability, and high biostability. Despite its therapeutic potential, clinical applications of this metalloenzyme have been constrained by suboptimal catalytic performance under physiological conditions, which is primarily attributed to insufficient intracellular Mg 2+ concentrations (typically
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
RNA Interference and Gene Delivery
DNA and Nucleic Acid Chemistry
Publication details
- Journal / source
- Biotechnology Journal
- Publisher
- Wiley
- Volume
- 21
- Issue
- 2
- Pages
- e70191–e70191
- Publication date
- 1 February 2026
- Language
- EN