3D Cryogel Composites of Modified Purine Derivative as Osteoinductive Agents for Bone Pathologies

Purines and their receptors play a role in the regulation of stem cell survival, proliferation, and differentiation. They are responsible for the osteogenic differentiation of stem cells by activating many signaling pathways and enhancing the expression of different osteogenic factors, including bone morphogenetic proteins, runt-related transcription factor 3, alkaline phosphatase (ALP), etc. Here, a 2,6,9-trisubstituted adenine derivative is reported, with negligible cytotoxicity and potent osteogenic potential as demonstrated with mouse myoblast (C2C12 cells) and murine preosteoblasts (MC3T3E1 cells). Osteogenic activity of the derivative (Compound 1) is supported by increased expression of ALP and enhanced calcium deposition observed with alizarin staining within cells. Thus, the reported adenine derivatives can serve as potential therapeutics for bone fractures as an injectable therapeutic opening avenue for cell-free therapy.