Expanding the Toolbox for Inducible Protein Expression With Automation‐enabled Generation of Glycomimetics
Abstract
Inducible protein expression is a cornerstone of many aspects of industrial and molecular biotechnological processes. However, limited availability of inducible transcription factors can reduce our ability to control expression at a population level. The design and synthesis of a powerful inducer containing a fucose is demonstrated to induce protein expression through the lac operon only in cells with the ability to selectively de-fucosylate them. Batch and automated continuous-flow processes are reported for the syntheses of both 2'-fucosyl isopropyl-β-D-thiogalactopyranose (2'F-IPTG) and isobutyl-C-galactoside (2'F-IBCG) mimics. Fucosylation of the inducer allowed for fucosidase-dependent expression of a reporter protein, providing an additional layer of control over inducible gene expression.
Fields of Study & Subjects
Carbohydrate Chemistry and Synthesis
Click Chemistry and Applications
Glycosylation and Glycoproteins Research
Publication details
- Journal / source
- Chemistry - A European Journal
- Publisher
- Wiley
- Volume
- 32
- Issue
- 10
- Pages
- e02010–e02010
- Publication date
- 7 January 2026
- Language
- EN