Synthesis and Early Assessment of Epothilone‐Derived Fluorescent Probes for Microtubule Imaging
Abstract
The synthesis of two bright epothilone-based fluorescent probes for microtubule imaging is described. Building on a tested strategy for the synthesis of epothilone-type structures, an analog of epothilone B with an extended C(6) substituent terminating in an alkyne group has been prepared. A Huisgen [3 + 2] cycloaddition of this analog with azide-functionalized derivatives of Alexa Fluor 488 and Janelia Fluor 646 gave bright fluorescent probes emitting in the green and far-red spectral range, respectively. Both probes bind to microtubules with high affinity and specifically label the microtubule network in A549 cells.
Fields of Study & Subjects
Cancer Treatment and Pharmacology
Microtubule and mitosis dynamics
Microbial Natural Products and Biosynthesis
Publication details
- Journal / source
- ChemMedChem
- Publisher
- Wiley
- Volume
- 21
- Issue
- 2
- Pages
- e202500947–e202500947
- Publication date
- 1 January 2026
- Language
- EN