Dual Ionizable Lipids (ALC0315 and SM102) in a Novel Lipid Nanoparticle Formulation Enhance In Vivo Delivery and Time‐Dependent Expression of Messenger RNA and Self‐Amplifying RNA

ChemMedChem 2026

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Authors: Dmitry Kunyk, Olga Vasileva, Mikhail Bespalov, Alina Sh. Dzharullaeva, Elena Mazunina, Anastasia Chubarova, Maxim Seliverstov, Denis Smirnykh, Daniil Shevyrev, Sofia Shtraus, Alena Kozlova, Elena Subcheva
+8 more Denis Kleymenov, Evgeniia Bykonia, Maxim V. Baranov, Denis Y. Logunov, Vladimir A. Gushchin, Roman A. Ivanov, Vasiliy V. Reshetnikov, Ivan A. Skvortsov
DOI: 10.1002/cmdc.70335
Cited by: 1
References: 42

Abstract

This study introduces a novel five-component LNP formulation incorporating ionizable lipid combination ALC-0315 and SM-102 with cholesterol, DSPC, and DMG-PEG-2000. LNP-5 was designed for efficient delivery of mRNA and saRNA. We employed a Design of Experiments approach and microfluidics to optimize the formulation parameters, achieving high encapsulation efficiencies (>80%) for both RNA types. Physicochemical characterization by dynamic light scattering confirmed a controlled size (80-120 nm) and low polydispersity (

Fields of Study & Subjects

RNA Interference and Gene Delivery Nanoparticle-Based Drug Delivery Advancements in Transdermal Drug Delivery

Publication details

Journal / source
ChemMedChem
Publisher
Wiley
Volume
21
Issue
11
Pages
e70335–e70335
Publication date
9 June 2026
Language
EN