Real‐time quality and safety monitoring of fruit juice using paper‐based platform

Abstract Food spoilage and safety concerns still remain critically challenging within the fruit juice industry, especially as conventional detection methods, though precise, are often too time‐consuming, costly, and reliant on centralized laboratories. This review critically evaluates the potential of paper‐based biosensors as a transformative solution for real‐time, on‐site monitoring of fruit juice quality. The inherent versatility of paper‐based biosensors, with the integration of both colorimetric and electrochemical detection methods, has attracted interest due to their portability, cost‐effectiveness, ease of use, and high analytical resolution. Their widespread application in environmental monitoring, medical diagnostics, and food safety assessment underscores their analytical versatility. We examine the underlying spoilage mechanisms, including microbial contamination, enzymatic browning, nonenzymatic browning reactions, and pesticide residues, alongside paper‐based biosensor designs and fabrication techniques. By offering near‐instant feedback, paper‐based biosensors support better decision‐making in juice production, distribution, and consumption. This review describes and highlights current advancements and challenges in sensitivity and quantification, as well as emerging trends such as multiplexing and digital integration. Collectively, this review summarizes the advantages and disadvantages of the paper as a matrix for sensors as well as its impact on their performance and applications, presents a short history of paper, and discusses fabrication methods and their functions.