Aggregation‐induced emission luminogens in in vitro diagnostics: Advancing biomarker detection in body fluids

Abstract Rapid, sensitive, and accurate detection of ingredients in body fluids is crucial for monitoring health status and diagnosing diseases. Fluorescent biosensors have emerged as a user‐friendly tool for biomarker detection, because of their high sensitivity, rapid response, and adaptability. Among the various signal probes used in fluorescent biosensors, aggregation‐induced emission (AIE) luminogens (AIEgens) stand out as a highly promising candidate owing to their unique property of enhanced emission in the aggregated or solid state. This review outlines the sensing mechanisms and design strategies of AIE probes for constructing AIE‐based fluorescent biosensors. It provides a comprehensive overview of recent advancements in AIE probes for detecting various ingredients in body fluids, including blood, urine, saliva, throat swabs, and sweat. The efficacy of AIEgen‐based fluorescent biosensors in these applications is highlighted, focusing on the design of probes, response mechanisms, and luminescence principles. Finally, the current challenges of AIEgen‐based biosensors and future research perspectives are discussed, aiming to inspire the development of AIE probes and improve the sensitivity and accessibility of AIEgen‐based fluorescent biosensors.