miR‐196b‐5p Targets TGFBR3 to Reinforce the Invasion and Migration of LUAD Cells

In this study, we investigated the underlying mechanisms of lung adenocarcinoma (LUAD) by focusing on miR-196b-5p as a research target, aiming to provide potential targets for LUAD treatment. Differentially expressed miRNAs and target mRNAs involved in this study were identified through bioinformatics analysis. miR-196b-5p and TGF-β receptor 3 (TGFBR3) mRNA were tested via qRT-PCR. Western blot (WB) analysis was undertaken to test the expression of TGFBR3 protein. Cell scratch-healing and transwell assays were employed to examine the invasive and migratory abilities of LUAD cells. Furthermore, the interaction between miR-196b-5p and its downstream target genes was confirmed through a dual luciferase assay. The results showed that miR-196b-5p was upregulated in LUAD cells, while TGFBR3 expression was significantly downregulated. High expression of miR-196b-5p facilitated the migratory and invasive capabilities of LUAD cells. Furthermore, dual-luciferase assays validated that TGFBR3 was a specific target of miR-196b-5p in LUAD cells. miR-196b-5p in LUAD cells targeted to downregulate TGFBR3 to enhance cell invasion and migration. In summary, our study reveals that miR-196b-5p targets and downregulates TGFBR3, thereby boosting the migration and invasion of LUAD cells.