Isolation of an Ammonium Excreting Actinomycetota, Microbacterium bengalense sp. nov. GB16_1_BI T , From Rice Rhizosphere

ABSTRACT A novel Gram‐stain positive bacterial strain, GB16_1_BI, with 70.3% G+C content was isolated from rice roots cultivated in the Madhyamgram field station of Bose Institute, West Bengal, India. GB16_1_BI is a nonmotile, nitrate reducing, ammonium releasing and plant growth promoting bacteria. The average nucleotide identity‐analysis of GB16_1_BI showed highest identity with Microbacterium enclense NIO‐1002 T (ANIb: 82.55%; ANIm: 87.51%) and Microbacterium phycohabitans KSW2‐29 T (ANIb: 82.53%; ANIm: 86.79%). Type strain genome server comparison of whole genome sequence of GB16_1_BI with genomes sequences for other species of the genus predicted that GB16_1_BI as a novel species with the digital DNA‐DNA hybridization values of < 30% with its closest phylogenetic relatives. The 16S rRNA sequence of GB16_1_BI showed 98.72% similarity with Microbacterium proteolyticum RZ36 T , 98.42% similarity with Microbacterium phycohabitans KSW2‐29 T and 98.43% with M. enclense NIO‐1002 T . Whole cell fatty acid analyses showed iso‐C16:0, anteiso‐C17:0 and anteisoC15:0 as the predominant fatty acids. The strain was designated as Microbacterium bengalense sp. nov GB16_1_BIT (=MTCC 13245=JCM 37885).