In Vitro Regeneration of Platycerium ridleyi Christ. Sporophytes via Homogenized Green Globular Bodies

This study aimed to improve the in vitro propagation method for Platycerium ridleyi Christ. Juvenile leaves from in vitro‐grown sporophytes were used as explants to induce green globular bodies (GGBs) on Murashige and Skoog (MS) medium supplemented with 5.37 μM α‐naphthaleneacetic acid (NAA). No significant discrepancy in GGB induction was observed between explants taken from the proximal or distal halves of leaves. Histological analysis revealed that the GGB surface was densely populated with meristematic cells, which formed compact clusters characterized by prominent nuclei and few vacuoles—features characteristic of GGB tissue. Inoculation of homogenized GGBs onto plant growth regulator (PGR)‐free medium demonstrated that a single inoculum of 0.05 g fresh tissue per Petri dish resulted in significantly higher regeneration (∼120 sporophytes per dish) compared to larger inocula of 0.1, 0.15, or 0.2 g ( p < 0.05). Regeneration was further increased by supplementing the culture medium with 2.5 g L −1 activated charcoal (AC), which significantly improved the regeneration process, resulting in a much higher yield of ∼360 sporophytes per dish. In contrast, the addition of polyvinylpolypyrrolidone (PVPP) or polyvinylpyrrolidone (PVP) had a comparatively limited effect. During acclimatization trials, sporophytes grown on a peat medium, with or without pre‐existing roots both displayed 100% survival.