Mitogenomes of Seven Malaysian Marine Gastropods Recovered Using Genome Skimming

Gastropods (Phylum: Mollusca, Class: Gastropoda) are the most diverse group of molluscs, comprising about 80% of classified species. Despite this, mitochondrial genome (mitogenome) data for Malaysian marine gastropods remain scarce. In this study, we analyzed the complete mitogenomes of seven Malaysian intertidal gastropods from three subclasses: Caenogastropoda ( Chicoreus brunneus , Indothais malayensis ), Neritimorpha ( Nerita undata ), and Vetigastropoda ( Astralium calcar , Lunella cinerea , Trochus radiatus , Turbo chrysostomus ). These mitogenomes, rich in A + T content (60.9%–69.4%), ranged from 15,586 to 17,661 base pairs. Comparative analysis revealed that Vetigastropoda possessed longer protein‐coding genes, rRNAs, and intergenic regions but fewer overlapping genes. The gene order in Neritimorpha and Vetigastropoda mostly aligned with the ancestral gastropod mitogenome, whereas Caenogastropoda showed notable reorganization. Vetigastropoda species exhibited tRNA gene duplications, such as additional trnG and trnE . Most tRNAs had typical cloverleaf secondary structures, except trnS, which lacked a dihydrouracil arm. Phylogenetic analysis placed each species within its respective subclass and suggested a close evolutionary relationship between Neritimorpha and Vetigastropoda. This study provides new mitogenomic data essential for understanding the evolutionary biology, systematics, and biodiversity of Malaysian marine gastropods.