Scalable Transfer of DNA Origami–Directed Nanoparticle Arrays onto Functional Surfaces Using Thermal Release Tape

ABSTRACT Precise integration of DNA origami nanostructures onto functional substrates is essential for advancing electronics, photonics, and biosensing. However, most surface‐assembly methods are highly sensitive to substrate type, limiting their broader applications. Here, we introduce a thermal release tape (TRT)‐assisted transfer method that enables efficient, large‐area printing of DNA origami‐guided gold nanoparticle (AuNP‐DNA origami) arrays from mica onto diverse surfaces. The method relies on differential adhesion: AuNP‐DNA origami arrays form strong electrostatic and hydrogen‐bonding interactions with 3‐aminopropyltriethoxysilane (APTES)‐functionalized receiver substrates, while the TRT adhesive weakens upon heating to 120°C, allowing release from the tape. Using this approach, well‐ordered AuNP‐DNA origami arrays were transferred onto silicon, Silicon carbide, and glass coverslips. Under optimized conditions, on average, ∼70% of the prepatterned domain area on mica was successfully transferred to silicon while preserving the designed lattice geometry; non‐optimized surface treatments or peeling conditions yielded lower transfer efficiencies, underscoring the need for process optimization. The TRT‐assisted process is simple, scalable, and material‐independent, avoiding lithographic patterning or substrate‐specific self‐assembly. This proof‐of‐concept study establishes a versatile platform for expanding substrate compatibility in DNA nanotechnology and provides a foundation for the large‐scale integration of nanoscale patterns into functional devices.