Label‐Free Fluorescence‐Based Enzyme Assays Using Supramolecular Host‐Guest Approaches

Small Methods 2026

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Authors: Zhe Zheng, Yan Xu, Ruina Liu, Yandong Zhang, Hanyu Lv, Wen‐Chao Geng, Chunju Li
DOI: 10.1002/smtd.202502355
Cited by: 3
References: 89

Abstract

Monitoring enzymatic reactions is crucial in enzymology, drug metabolism, and bioengineering. Supramolecular tandem enzyme assays (STA), based on indicator displacement assays, leverage the differential binding affinities of supramolecular hosts for enzymatic substrates and products. STA enables label-free, real-time, and rapid monitoring of enzymatic reactions, offering advantages such as simplicity, high sensitivity, and strong specificity. STA has been extensively employed to determine enzymatic kinetic parameters, screen inhibitors, diagnose biomarkers, and analyze enantiomeric excess. This review summarizes recent advancements in STA strategies across various enzyme types and provides current challenges and perspectives for future development.

Fields of Study & Subjects

Molecular Sensors and Ion Detection Luminescence and Fluorescent Materials Click Chemistry and Applications

Publication details

Journal / source
Small Methods
Publisher
Wiley
Volume
10
Issue
5
Pages
e02355–e02355
Publication date
17 February 2026
Language
EN