Cover Feature: Cas12Fold Accurately Predicts Cas12 Nuclease Structures to Enable Structure‐based Genome‐editing Engineering (Small Struct. 2/2026)

Small Structures 2026 Open access

Read full text
Authors: Feng Xu, Zilong Zhao, Chang Liu, Meixia Yu, Ke Li, Yilin Jing, Peiyang Li, Beibei Xin, Jian Chen, E Lizhu, Jinsheng Lai, Zhijia Yang
+1 more Hainan Zhao
DOI: 10.1002/sstr.70313
Cited by: 0

Abstract

Structure-Based Genome-Editing Engineering In their Research Article (10.1002/sstr.202500674), Zhijia Yang, Hainan Zhao, and co-workers present Cas12Fold, a deep-learning framework tailored to Cas12 nucleases, that uses evolutionary signals and iterative structure-based alignment to resolve conformational complexity. This method outperforms general models in predicting key domains and states, accurately modeling enzymes such as Casλ. Models guide mutagenesis, optimizing Cas12j.4 efficiency and enabling CRISPR–Cas12 engineering.

Fields of Study & Subjects

CRISPR and Genetic Engineering Protein Structure and Dynamics Enzyme Structure and Function

Publication details

Journal / source
Small Structures
Volume
7
Issue
2
Publication date
1 February 2026
Language
EN