Cell Painting Protocol to Characterize Morphological Profiles of Large Compound Collections Using the EU-OPENSCREEN Library

Methods in molecular biology 2026 Book chapter Open access

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Authors: Christopher Wolff, Martin Neuenschwander, Michelle Müller, Astrid Mühl, Jens Peter von Kries, Christopher Schmied, Edgar Specker
DOI: 10.1007/978-1-0716-4985-5_4
Cited by: 0
References: 21

Abstract

Morphological profiling with the Cell Painting assay started a new era in phenotypic screening approaches by harnessing comprehensive morphological profiles of cellular perturbations. This enables an unbiased characterization of the effects of small chemical compounds. We established and extensively validated a Cell Painting protocol to screen the compound libraries of the European initiative EU-OPENSCREEN ( www.eu-openscreen.eu ) in order to find robust and reproducible links between the known target or the pathway mechanism of each compound to the phenotypic profile. In this chapter, we describe the Cell Painting procedure, image analysis, and the downstream data processing procedures.

Fields of Study & Subjects

Cell Image Analysis Techniques Single-cell and spatial transcriptomics Cellular Mechanics and Interactions

Publication details

Journal / source
Methods in molecular biology
Volume
2989
Pages
73–91
Publication date
1 January 2026
Language
EN
Document type
Book chapter