Time-Series Neutron Tomography of Rhizoboxes

Abstract This study investigates the feasibility of tilt-series neutron tomography for analyzing rhizoboxes used in root-soil interaction studies. Traditional neutron imaging methods are limited by constrained root growth volumes and poor penetration in moist soil. Using a vertical acquisition axis, the tilt-series approach avoids constraints typical of laminography. This method allows simultaneous radiographic and tomographic data acquisition, enhancing time resolution and providing detailed insights into root networks and soil water content. Experiments involved scanning six-week-old maize plants in rhizoboxes filled with sand. Results show that tilt-series tomography can effectively reconstruct root networks despite some artifacts from the missing wedge. While the tilt-series tomographic data qualitatively reveal water distribution changes, radiographic data remain essential for quantitative analysis. This approach demonstrates the potential for dynamic root-soil interaction studies, offering a valuable tool for agricultural and environmental research by providing comprehensive insights into the rhizosphere.