ISCEV extended protocol for the photoreceptor directed ERG using full-field silent substitution stimuli

The International Society for Clinical Electrophysiology of Vision (ISCEV) Standard for full-field electroretinography (ERG) describes a minimum procedure, but encourages more extensive testing. This ISCEV extended protocol describes an extension to the full-field ERG Standard, namely the ERG responses to silent substitution stimuli that are directed at the response modulation of single photoreceptor types. The method can be used to detect selective defects in distinct photoreceptor types and post-receptoral retinal pathways. This document, based on existing literature, provides protocols for recording and analyzing the responses to a single class of photoreceptor directed stimuli. For each photoreceptor type (rods, L-, M- and S-cones), optimal luminance conditions and temporal frequencies for Ganzfeld recordings are provided. The exact stimulus conditions depend on the spectral properties of the stimulator. An accompanying technical report describes an app that is meant to provide support in calculating stimuli for individual stimulators.