Development of in vitro skin corrosion test using KeraSkin™

Skin corrosion and irritation are critical local toxicological endpoints associated with chemical exposure. OECD has approved the use of the Reconstructed Human Epidermis (RhE) model, KeraSkin™, as me-too in vitro skin irritation test of OECD Test Guideline (TG) 439. Here, we explored the applicability of KeraSkin™ as a me-too test method for in vitro skin corrosion test OECD TG 431, to expand its validated use beyond skin irritation test. Thirty reference chemicals, including Category 1A, 1B/1C, and non-corrosive substances as described in OECD TG 431 performance standards (PS), were tested. Test chemicals were applied for 3 or 60 min, and cell viability (CV) was measured using MTT assay. Classification criteria were based on viability thresholds, wherein