Evaluation of Marsilea minuta Linn. for wound healing activity through the excision wound model

The present study investigates the wound healing activity of Marsilea minuta Linn. extract on Wistar rats by using the excision wound model. After authentication at the Central Regional Botanical Survey Center, the plant extract was performed using 80% methanol and evaluated thoroughly regarding phytochemical screening. Prominent phytoconstituents present were detected as alkaloids, flavonoids, tannins, and phenolic compounds, which were assessed in comparison by employing standard analytical techniques. The methanolic extract was added at different concentrations: 10%, 20%, and 40% within the ointments, and their wound healing potential was evaluated. The in vivo experiments showed significant wound contraction, especially with the ointment form of the 20% extract, that was superior in healing rate and comparable to the standard drug, povidone iodine. It decreased the period of epithelialization and enhanced the activities of antioxidant enzymes, such as SOD, CAT, and GSH, and reduced lipid peroxidation (TBARS). Histopathological observation also supported these findings, as reduced inflammation and increased formation of granulation tissue were found in the treated groups. From the study, it can be concluded that Marsilea minuta Linn. contains significant wound healing properties and rich phytochemical content together with antioxidant mechanisms, which could make this plant a very promising candidate in natural wound care therapies.