Comparative analysis of anti-MICA scFv affinities: Insights from three label-free biophysical methods and biological validation

Biotechnology Reports 2026 Open access

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Authors: Karen Toledo-Stuardo, Nicolás Fehring, Homero Gómez-Velasco, Rodrigo Sierpe, Daniel Guerra, Douglas J. Matthies, Yuneisy Guerra, Fabiola González‐Herrera, Mauricio González, Ivo Campos, Samantha Tello, María José Garrido
+11 more Gonzalo Vásquez, Jose Rodríguez-Siza, Mauricio Vergara, Carolina Ribeiro, Gerald Zapata, Soledad Bollo, Enrique Garcia-Hernandez, Denis Fuentealba, Norma A. Valdez‐Cruz, Claudia Altamirano, M.C. Molina
DOI: 10.1016/j.btre.2026.e00955
Cited by: 2
References: 55

Abstract

) varied depending on the method used, but SPR provided the most precise discrimination. Flow cytometry confirmed stronger binding of the WT scFv to MICA-expressing gastric cell lines, which was consistent with the results obtained using SPR. Simulations indicated greater conformational stability and more favourable antigen contacts for the WT scFv. Together, these results highlight the value of orthogonal measurements for robust affinity assessment and support WT as a promising lead for further development. This benchmark provides practical guidance for selecting affinity assays in discovery pipelines.

Fields of Study & Subjects

Immune Cell Function and Interaction Phagocytosis and Immune Regulation interferon and immune responses

Publication details

Journal / source
Biotechnology Reports
Publisher
Elsevier BV
Volume
50
Pages
e00955–e00955
Publication date
28 February 2026
Language
EN