Holotranscobalamin-based algorithm for the identification of macro-vitamin B12 in analytical hypercobalaminemia

Clinica Chimica Acta 2026 Open access

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Authors: Patricia Fernández-Tomás, Leyre Ruiz, Estíbaliz Alegre, Álvaro González
DOI: 10.1016/j.cca.2026.121085
Cited by: 1
References: 31

Abstract

BACKGROUND: Interference by vitamin B12 (B12) macrocomplexes is common but underappreciated in clinical practice. When unexpected hypercobalaminemia is detected, analytical interference must be considered. Holotranscobalamin (HoloTC) reflects the biologically active B12 fraction, but the impact of B12 macrocomplexes (M-B12) on HoloTC immunoassay performance remains unclear. METHODS: We collected 528 adult serum samples. Total B12 and HoloTC were measured by commercial immunoassays. M-B12 presence was defined as B12 recovery after polyethylene glycol precipitation (PEG)

Fields of Study & Subjects

Folate and B Vitamins Research Porphyrin Metabolism and Disorders Iron Metabolism and Disorders

Publication details

Journal / source
Clinica Chimica Acta
Publisher
Elsevier BV
Volume
590
Pages
121085–121085
Publication date
15 May 2026
Language
EN