Continuum architecture dynamics of vesicle tethering in exocytosis

Cell 2026 Open access

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Authors: Marta Puig-Tintó, Sebastian Ortiz, Sasha Meek, Raffaele Coray, Laura Betancur, Altair C. Hernández, Anna Castellet, Eric M. Kramer, Philipp Hoess, Markus Mund, Andrés Molina-Ribagorda, Mercè Izquierdo-Serra
+6 more Baldo Oliva, Alex de Marco, Jonas Ries, Daniel Castaño‐Díez, Carlo Manzo, Oriol Gallego
DOI: 10.1016/j.cell.2025.11.038
Cited by: 2
References: 84

Abstract

Essential for eukaryotes, multiple copies of the exocyst complex tether each secretory vesicle to the plasma membrane (PM) in constitutive exocytosis. The exocyst higher-order structure (ExHOS) that coordinates the action of these multiple exocysts remains unexplored. We integrated particle tracking, super-resolution microscopy, and cryo-electron tomography to time-resolve the continuum conformational landscape of the ExHOS and to functionally annotate its different conformations. We found that 7 exocysts form a flexible ring-shaped ExHOS that tethers vesicles at

Fields of Study & Subjects

Cellular transport and secretion Lipid Membrane Structure and Behavior Bacterial Genetics and Biotechnology

Publication details

Journal / source
Cell
Publisher
Elsevier BV
Volume
189
Issue
4
Pages
1170–1184.e22
Publication date
16 January 2026
Language
EN