Uncovering spatially resolved functional genomics with CRISPR screen sequencing

Cell 2026 Open access

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Authors: Haorui Zhang, Zongxu Zhang, Peiyu Wang, Tian Xu, Xiaoyu Chen, Yanping Zhao, Siyu Lin, Wenjie Cai, Pengfei Ren, Ce Luo, Peng Zhang, Yunfeng Wang
+9 more Sen Hou, Yahui Zhao, Hu Zeng, Zhihua Liu, Cunyu Wang, Zhidong Gao, Yu Feng, Deng Pan, Zexian Zeng
DOI: 10.1016/j.cell.2026.04.049
Cited by: 6
References: 137

Abstract

T cells, we revealed Cd44's role in regulating spatial phenotypes by interacting with Spp1 on macrophages. We also demonstrated the model of the transcription factor-chemokine receptor axis coupling cell states with chemotaxis. SPAC-seq and TARDIS provide an effective framework to study spatially resolved functional genomics and pathways across diverse biological and disease contexts.

Fields of Study & Subjects

Single-cell and spatial transcriptomics CRISPR and Genetic Engineering Bioinformatics and Genomic Networks

Publication details

Journal / source
Cell
Publisher
Elsevier BV
Volume
189
Issue
15
Pages
4594–4618.e48
Publication date
26 May 2026
Language
EN