Diastereomer separation of phosphorothioate oligonucleotides via conformationally stabilizing ion pairs in reversed-phase liquid chromatography

Journal of Chromatography A 2026 Open access

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Authors: Peiyu Zhang, Tian Wang, Suzhen Jiang, Qiancheng Li, Weijia Kong, Kexin Yang, Haijiang Nan
DOI: 10.1016/j.chroma.2026.467137
Cited by: 1
References: 31

Abstract

) measurements. To best of our knowledge, this is the first time that ion pairing reagents with conformation-stabilizing effect are used for both single- and double-stranded oligonucleotide analysis. This work enriches the analytical toolkit for oligonucleotide characterization and may enable batch-to-batch diastereomer distribution monitoring in synthetic phosphorothioate oligonucleotides.

Fields of Study & Subjects

Analytical Chemistry and Chromatography Chemical Synthesis and Analysis Origins and Evolution of Life

Publication details

Journal / source
Journal of Chromatography A
Publisher
Elsevier BV
Volume
1783
Pages
467137–467137
Publication date
27 May 2026
Language
EN