Engineering yeast metabolism for robust bioproduction under industrial conditions

Yeast industrial bioproduction is often constrained by stress, substrate variability, and metabolic imbalance. This review provides a critical overview of recent advances in engineering yeast metabolism for robust performance under industrial conditions, increasingly defined by the use of renewable and heterogeneous feedstocks, which impose additional physiological and metabolic challenges. We highlight strategies that have gained particular relevance in the current literature, including the engineering of redox homeostasis and central carbon metabolism, detoxification and stress mitigation pathways, and the reduction of metabolic burden. The increasing contribution of systems biology and multi-omics, alongside dynamic and synthetic regulatory strategies, is also addressed. Finally, we explore the potential of naturally robust yeast chassis and outline future directions for resilient, sustainable, and scalable bioproduction systems.