Transcriptomic dataset of RAW264.7 murine macrophages pretreated with 9-methoxycanthin-6-one under poly(I:C)-TLR3 stimulation

This dataset presents RNA sequencing (RNA-seq) data from RAW264.7 murine macrophages pretreated with 9-methoxycanthin-6-one, a canthin-6-one–type alkaloid isolated from Eurycoma longifolia Jack, and subsequently stimulated with polyinosinic:polycytidylic acid [poly(I:C)], a synthetic double-stranded RNA analog that activates TLR3-mediated antiviral signaling. RAW264.7 cells were pretreated with 9-methoxycanthin-6-one (30 μM) for 30 min and then exposed to poly(I:C) (20 μg/mL) for 6 h. Total RNA was extracted, quality-checked, and sequenced on the Illumina platform to generate paired-end reads. Differential expression analysis and functional annotation were performed to profile genes responsive to 9-methoxycanthin-6-one treatment under poly(I:C) stimulation. The dataset includes normalized expression matrices, lists of upregulated and downregulated genes, and pathway enrichment outputs in standard formats. These data provide a reference resource for understanding the transcriptomic responses of macrophages to natural alkaloid treatment during viral-mimetic immune activation. The dataset can be reused to compare host antiviral transcriptional responses across TLR3-related pathways, evaluate macrophage activation markers, or integrate with other E. longifolia bioactive compounds.