Detection of stipe rot disease in Morchella sextelata based on enhanced Fourier transform infrared spectroscopy

Timely detection of stipe rot disease is crucial for ensuring the yield and quality of Morchella sextelata (morel). In this study, we propose a volatile-enhanced Fourier transform infrared (FTIR) spectroscopy for detecting stipe rot disease. The signature volatiles of disease were analyzed and identified by long-path enhanced FTIR spectroscopy. Moreover, the enrichment-enhanced spectral properties of the signature volatile were analyzed using a metal–organic framework (MOF) enrichment-enhanced FTIR spectroscopy. A multivariate quantitative model and an identification model for the stipe rot disease were established based on enrichment-enhanced spectral characteristics combined with chemometric methods. The results show that ammonia is the signature volatile, and the quantitative model demonstrates a superior ability to quantify ammonia. The support vector machine enables the identification of disease degree and achieves a recognition accuracy of 98.75%. The volatile-enhanced FTIR technique provides a feasible method for the rapid and non-destructive detection of stipe rot disease in morel. • Long-path enhanced FTIR spectroscopy identifies signature volatiles of stipe rot. • MOF enrichment-enhanced FTIR spectroscopy can sense key volatile biomarker. • The ammonia content levels of four disease stages of stipe rot are assessed. • Enhanced FTIR technology allows non-destructive detection of morel stipe rot disease.