Chemical and genetic knockout identifies KCNQ2 as the principal background voltage-gated potassium current in human embryonic kidney 293 cells

Heart Rhythm 2026 Open access

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Authors: Matthew J. O’Neill, Tao Yang, Yuko Wada, Marcia A. Blair, Andrew Michael Glazer
DOI: 10.1016/j.hrthm.2026.01.043
Cited by: 1
References: 4

Abstract

Patch-clamp electrophysiology has transformed functional assessment of genotype-phenotype relationships in inherited arrhythmia syndromes. These methods have traditionally relied on in vitro analysis of ion channels transiently or stably expressed in heterologous cell models. Voltage-gated potassium channels have drawn particular attention due to their role in disorders such as Long QT Syndrome (e.g., LQT1: KCNQ1; LQT2: KCNH2; LQT5: KCNE1; LQT7/Andersen-Tawil Syndrome: KCNJ2).1 Chinese Hamster Ovary (CHO) cells and Human Embryonic Kidney (HEK293) cells are widely used models for functional studies of candidate channelopathy-associated variants.

Fields of Study & Subjects

Cardiac electrophysiology and arrhythmias Ion channel regulation and function Ion Transport and Channel Regulation

Publication details

Journal / source
Heart Rhythm
Publisher
Elsevier BV
Volume
23
Issue
7
Pages
e1183–e1185
Publication date
3 February 2026
Language
EN