LncRNA SLC16A1-AS1 cooperates with NSUN2 to stabilize GRP78 mRNA via m5C modification in gastric cancer

iScience 2026 Open access

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Authors: Beiyao Zheng, Wenjing Zhao, Yan Jin, Wentao Sun, Yong Li, Qi Zhang, Yue Li, Jin WANG, Dong Chen, Ke Ren, Wei Zhang, Yuanyuan Chen
+4 more Li Yuan, Xue Jun Wang, Fen Yang, jinfei chen
DOI: 10.1016/j.isci.2025.114614
Cited by: 0
References: 53

Abstract

. Mechanistically, SLC16A1-AS1 interacted with the 5-methylcytosine (m5C) methyltransferase NOL1/NOP2/SUN (NSUN2), enhancing m5C modification of GRP78 mRNA, which stabilized the transcript and increased GRP78 protein levels. Rescue experiments demonstrated that GRP78 overexpression reversed the proliferation-inhibitory effect of SLC16A1-AS1 depletion. These findings reveal that SLC16A1-AS1 drives GC cell proliferation via NSUN2-mediated m5C modification of GRP78 mRNA, suggesting a potential target for GC diagnosis and therapy.

Fields of Study & Subjects

Cancer-related molecular mechanisms research RNA modifications and cancer RNA Research and Splicing

Publication details

Journal / source
iScience
Volume
29
Issue
2
Pages
114614–114614
Publication date
18 January 2026
Language
EN