Phage-based diagnostic assay for melioidosis using multiplex PCR targeting Burkholderia phage terminase genes

iScience 2026 Open access

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Authors: Patoo Withatanung, Muthita Vanaporn, Narisara Chantratita, Sorujsiri Chareonsudjai, Veerachat Muangsombut, Sujintana Janesomboon, Thatchanon Asawalertsaeng, Martha R. J. Clokie, Edouard E. Galyov, Sunee Korbsrisate
DOI: 10.1016/j.isci.2026.114831
Cited by: 0
References: 43

Abstract

phage terminase genes from serum samples, resulting in increased sensitivity under low-bacteremia conditions. To our knowledge, this study reports a phage-based PCR assay that detects phage DNA directly from melioidosis patients' blood, representing a shift from molecular pathogen-based to phage-based diagnostics. This approach not only improves sensitivity but also opens avenues for integrating phage biology into the diagnostic landscape of infectious diseases.

Fields of Study & Subjects

Burkholderia infections and melioidosis Bacteriophages and microbial interactions Biosensors and Analytical Detection

Publication details

Journal / source
iScience
Volume
29
Issue
3
Pages
114831–114831
Publication date
30 January 2026
Language
EN