Functional assessment of a convergent gene network underlying allergic diseases and IgE

F095 We proposed a novel network-based approach that integrated a comprehensive human interactome with the results of genome-wide association studies of two different omics with two different phenotypes (GWAS of allergic diseases (AD) and EWAS of Immunoglobulin E (IgE) levels). This study aimed to identify AD-associated genes and IgE-associated genes, having close proximity in the human interactome, and to elucidate the associated biological mechanisms. F095 In-depth functional assessment of a set of 139 AD and IgE genes, with direct cross-trait connections in the interactome, revealed their involvement in both type 2 and non-type 2 immune responses. This analysis also emphasized the role of IgE in both allergic and antiviral responses. F095 New functionally interacting genes that influence AD and IgE levels were identified. These genes are involved in various biological processes including host defense against pathogens, ubiquitination, regulation of gene expression, inflammation and neuroimmune interactions.. The relationships between allergic diseases (AD) and Immunoglobulin E (IgE) levels are complex. To identify genes associated with AD and IgE, having close proximity in the interactome, and to elucidate the associated biological mechanisms. We integrated a comprehensive interactome with data from genome-wide (GWAS) and epigenome-wide (EWAS) association studies of AD and IgE levels, respectively. We used the SigMod algorithm to identify gene modules enriched in AD- and IgE-associated signals. We performed a connectivity analysis of these two modules to characterize direct cross-trait connections. Finally, we conducted an in-depth functional analysis of the directly-connected AD and IgE genes. We identified gene modules that were significantly associated with AD and IgE, respectively ( P