Exploring the soothing and reparative effects of Prinsepia utilis Royle oil based on network pharmacology and experimental validation

To evaluate the soothing and reparative effects of Prinsepia utilis Royle oil in cosmetics, network pharmacology was employed to analyse the common targets of P. utilis Royle oil and skin inflammation and repair. Network analysis and enrichment analyses were conducted to identify potential possible pathways and targets. Subsequently the soothing and reparative effects of P. utilis Royle oil were verified through cellular and zebrafish experiments by measuring relevant indicators. The results indicated that AKT1, EGFR, TNF, LCK, and CDC42 were the primary gene targets associated with skin inflammation and repair. The oil demonstrated soothing and reparative properties related to kinase binding, peptide regulation, leukocyte regulation, hydrolase activity and T cell receptor signaling pathway. At volume concentrations of 0.1%, 0.05%, and 0.01%, the oil significantly inhibited the secretion of nuclear factor NF-κB and histamine in mouse mast-cell tumour P815 cells, and it also significantly suppressed the secretion of tumour necrosis factor TNF-α in zebrafish. Furthermore, it markedly promoted the repair and expression of OCLN in zebrafish with caudal fin injury ( P < 0.05). In conclusion, from the perspectives of network pharmacology and experimental validation, the findings demonstrate that P. utilis Royle oil possesses notable soothing and reparative effects.