Development of a greener flow-based analytical tool for the expeditious determination of total soluble proteins
Abstract
Protein hydrolysates are increasingly used in feed, underscoring the need for analytical tools for the rapid, reliable determination of total protein. This study revisited a merging-zones flow-based spectrophotometric method for minimizing reagent consumption and employs the Biuret reaction for the quantification of total soluble protein in by-products hydrolysates. The analytical method was optimized across different physical and chemical parameters such as: flowrate, reactor length, sample injection volume and reagents concentration. The use of different matrices relevant in the hydrolysis processes (acetate, phosphate, and hydrochloric acid) showed no significant interference (
Fields of Study & Subjects
Protein Hydrolysis and Bioactive Peptides
Analytical chemistry methods development
Advanced Proteomics Techniques and Applications
Publication details
- Journal / source
- Journal of Food Composition and Analysis
- Publisher
- Elsevier BV
- Volume
- 154
- Pages
- 109178–109178
- Publication date
- 21 April 2026
- Language
- EN