Active peptides pursuit from complicated matrices via tracing ligand-protein complex with MS/MS: Chaperone screening for lysozyme as a pilot study
Abstract
• A refined workflow was designed for high-throughput screening of active peptides in complicated matrices. • Confidence was enhanced by directly capturing peptide-protein complex in MS 1 , assisted by MS 2 . • Lysozyme (Lyz) and Bacillus licheniformis fermentation broth were utilized for utility illustration and justification. • Fifteen potential peptides were detected, of which bacitracins A and B3 (Bacs A and B3) were annotated. • The interactions between Lyz and Bac A dramatically advanced the antibacterial performance of Lyz.
Fields of Study & Subjects
Antimicrobial Peptides and Activities
Advanced Proteomics Techniques and Applications
Mass Spectrometry Techniques and Applications
Publication details
- Journal / source
- Journal of Pharmaceutical Analysis
- Volume
- 16
- Issue
- 7
- Pages
- 101586–101586
- Publication date
- 12 February 2026
- Language
- EN