Expression and signaling patterns of CD180/MD-1 and CD150 in Chronic Lymphocytic Leukaemia (CLL) cells

cells favored p38MAPK signaling, indicating context-dependent modulation of downstream pathways. Notably, stimulation via CD150 alone induced phosphorylation of both AKT and p38MAPK in specific subsets, confirming its independent signaling capacity. In synchronized MEC-1 cells, CD180 and MD-1 expression peaked at 48 h, coinciding with enhanced AKT phosphorylation upon combined CD180/CD150 stimulation. These data suggest that the temporal dynamics of CD180 expression modulate the outcome of CD150-driven signaling, indicating a tightly regulated and interdependent interaction. Collectively, these findings define a CD180/MD-1/CD150 signaling axis that underlies the intracellular signaling heterogeneity in CLL. The relative expression balance between CD180 and CD150 may steer cells toward pro-survival or stress-associated signaling, providing novel mechanistic insight into CLL biology and identifying candidate biomarkers or therapeutic targets.