Targeting DNMT1 restores PTEN expression in colorectal cancer cells

Molecular and Cellular Probes 2026 Open access

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Authors: Muhamed A. El Nobey, Abdulkader M. Shaikh Omar, Ali Zari, Amani Alharthi, Maha Ali Alghamdi, Eman Hillal Althubaiti, Majed Al-Shaeri, Tahani Bakhsh, Salim M El Hamidy, Ashwaq H. Batawi, Amaal Hassan
DOI: 10.1016/j.mcp.2026.102074
Cited by: 1
References: 39

Abstract

values of 12.38 ± 0.98 μM and 10.04 ± 0.98 μM, respectively. Treatment with 5-aza-dC induced significant PTEN re-expression and DNMT1 downregulation at both the mRNA and protein levels. Methylation-specific PCR (MSP-PCR) suggested reduced PTEN promoter methylation following treatment. Additionally, miR-29a-3p expression was significantly upregulated. These findings suggest that 5-aza-dC restores PTEN expression in association with DNMT1 suppression and PTEN promoter demethylation, while miR-29a-3p upregulation may reflect an associated epigenetic response requiring further mechanistic validation.

Fields of Study & Subjects

PI3K/AKT/mTOR signaling in cancer Angiogenesis and VEGF in Cancer Inflammatory mediators and NSAID effects

Publication details

Journal / source
Molecular and Cellular Probes
Publisher
Elsevier BV
Volume
88
Pages
102074–102074
Publication date
22 May 2026
Language
EN