Comparative analysis of next-generation sequencing methods for whole-genome sequencing of seasonal influenza viruses

Infection Genetics and Evolution 2026 Open access

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Authors: Jin Sun No, Ji Yeong Noh, Chae Young Lee, Il‐Hwan Kim, Nam-Joo Lee, Jee Eun Rhee, Eun-Jin Kim
DOI: 10.1016/j.meegid.2026.105952
Cited by: 1
References: 20

Abstract

Influenza viruses are segmented negative-sense RNA viruses with high genetic variability, causing seasonal epidemics and pandemics. Whole-genome sequencing from clinical specimens is often hindered by low viral loads and host background, necessitating enrichment-based next-generation sequencing (NGS). In this study, we compared amplicon- and probe capture-based NGS using 26 influenza-positive clinical specimens representing A(H1N1), A(H3N2), and B/Victoria lineages, stratified by cycle threshold (Ct) (

Fields of Study & Subjects

Influenza Virus Research Studies Genomics and Phylogenetic Studies Respiratory viral infections research

Publication details

Journal / source
Infection Genetics and Evolution
Publisher
Elsevier BV
Volume
141
Pages
105952–105952
Publication date
23 April 2026
Language
EN