The establishment of TaqMan qPCR method for the identification and quantification of Amynthas aspergillum originated medicinal products

Microchemical Journal 2026 Open access

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Authors: Shuchen Duan, Wenjuan Zhang, Zhe Wu, Xianlong Cheng, Siyu Ma, Ping Li, Feng Wei
DOI: 10.1016/j.microc.2026.116827
Cited by: 2
References: 32

Abstract

“Guang Dilong” (GDL, Amynthas aspergillum ), a pharmacopeial “DiLong” (DL, Pheretima ) source in traditional Chinese medicine, faces widespread adulteration of various earthworm species. To enable specific and precise quantification of GDL in dried and highly processed medicinal products, a TaqMan qPCR assay targeting species-specific (including the newly classified subgroup of GDL) and earthworm-universal sequences with short amplicons (

Fields of Study & Subjects

Identification and Quantification in Food Leech Biology and Applications Invertebrate Taxonomy and Ecology

Publication details

Journal / source
Microchemical Journal
Publisher
Elsevier BV
Volume
221
Pages
116827–116827
Publication date
6 January 2026
Language
EN