A brief guide to the in vivo chemogenetic cell ablation approach in zebrafish

Molecules and Cells 2026 Open access

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Authors: Eun-Ji Lee, Jae-Geun Lee, Jae-Geun Lee, Jeong-soo Lee, Jeong-soo Lee
DOI: 10.1016/j.mocell.2026.100310
Cited by: 0
References: 12

Abstract

In vivo cell ablation technologies are essential tools for understanding biological processes within living animal models. The nitroreductase (NTR)/metronidazole system enables highly effective spatiotemporal cell ablation. Using transgenic zebrafish that combine NTR2.0 with the QF3/QUAS binary gene expression system, conditions to achieve efficient cell type-specific chemogenetic ablation were optimized. This approach provides a versatile in vivo platform for investigating developmental processes and regeneration, as well as for disease modeling and drug discovery.

Fields of Study & Subjects

Zebrafish Biomedical Research Applications Congenital heart defects research Microtubule and mitosis dynamics

Publication details

Journal / source
Molecules and Cells
Volume
49
Issue
2
Pages
100310–100310
Publication date
5 January 2026
Language
EN