A practical guideline for in vivo bioluminescence imaging
Abstract
Bioluminescence imaging (BLI) using luciferase-expressing cancer cells is a widely adopted method for noninvasive, real-time monitoring of tumor growth, metastasis, and therapeutic responses in vivo. Here, we summarize key considerations for effective implementation of BLI, including luciferin administration and data quantification. We also address common technical challenges and provide practical troubleshooting strategies to support the use of BLI in preclinical tumor models.
Fields of Study & Subjects
bioluminescence and chemiluminescence research
Optical Imaging and Spectroscopy Techniques
Biofield Effects and Biophysics
Publication details
- Journal / source
- Molecules and Cells
- Volume
- 49
- Issue
- 5
- Pages
- 100334–100334
- Publication date
- 23 February 2026
- Language
- EN