A biomimetic physical-biological coupled bone organoid 3D culture system for bone repair

The osteogenic and angiogenic capabilities of bone defect implants affect the regeneration and repair of bone tissue. This study introduces a novel organoid material that simulates the physical and biological characteristics of bone. Hydroxyapatite was used as the implant material and was printed in a graded manner using 3D printing to form a scaffold resembling bone trabeculae. Adding Gel-Ma hydrogels with extracellular matrix-like structures to the scaffold provides a biomimetic 3D culture environment for osteoblasts and vascular progenitor cells on the scaffold, making it closer to the human extracellular environment. The intrinsic physical parameters have been pre-calculated, proving that the material has a biomimetic morphological design. Through the experiments in vitro and in vivo, it has been demonstrated that the combination of the newly developed 3D culture system, the synergistic enhancement of vascularization within material, and the acceleration of bone repair through morphology biomimetics and biology present a promising platform for bone repair. In addition, we also design a quantitative-training deep learning model of multimodal data fusion, feature extraction, and key factor analysis, which closely relies on its complementary design and complex fusion mechanism to find the potential relationship between data and images.