Folate-targeted chitosan-coated Fe3O4 mesoporous nanoparticles for synergistic chemo-hyperthermia in breast cancer

Mesoporous nanoparticles that are magnetized have also been discussed as viable systems in combined chemo-hyperthermia delivery; nevertheless, a challenge of attaining high heating rates and efficient delivery of drugs to the targets has been a major challenge. This paper has produced folate-functionalized chitosan-coated magnetic mesoporous nanoparticles (CF-Doc-MMNPs) used in synergistic treatment of triple-negative breast cancer. The central composite design was used to optimize the formulation to obtain improved magnetization, measured particle size and efficient drug release. Optimized nanoparticles were found to have a homogeneous core-shell morphology with a mean hydrodynamic diameter of approximately 256 nm and a large surface area (approximately 150 m 2/g). A high specific absorption rate (SAR) of therapeutic hyperthermic temperatures was reached through magnetic characterization which established superparamagnetic behavior and the system had high specific absorption rate (SAR) of 113.62 W/g. The nanoparticles demonstrated that they had pH-responsive and sustained drug release, and the release was enhanced in the presence of acidic tumor conditions. In vitro cytotoxicity analyses of MDA-MB-231 cells showed that CF-Doc-MMNPs (LC 50 = 4.190 ug/mL) had a high anticancer activity due to the synergistic of magnetic hyperthermia and targeted drugs delivery in vitro. The current research is a multifunctional nanoplatform with high heating efficiency, controlled release, and folate-mediated targeting which could be useful in chemo-hyperthermia of aggressive breast cancer.