Effects of dietary inclusion of black cumin seed (Nigella sativa L.) meal on meat quality, hematology, and serum biochemical and antioxidant status of broiler chickens

This study evaluated the effects of dietary inclusion of black cumin seed meal (BCSM) on meat quality, hematology, and serum biochemistry, and antioxidant status of broiler chickens. A total of 144 one-day-old Cobb 500 chicks were randomly assigned to four dietary treatments containing 0%, 1.5%, 3%, and 4.5% BCSM in a completely randomized design with three replicates of twelve chicks each. Iso-nitrogenous and iso-energetic diets were provided over a 45-day of experimental period. Meat quality was assessed by chemical composition, physical quality traits and sensory attributes. Blood samples were collected to determine the hematological, serum biochemical and antioxidant parameters. Results indicated that BCSM inclusion, particularly at 3% and 4.5%, significantly (p < 0.05) improved meat quality by enhancing color, sensory traits, drip loss, cooking loss, and chemical composition of both breast and thigh meat. White blood cell count increased significantly (p < 0.05) at 4.5% inclusion level, while other hematological parameters were unaffected. Serum biochemistry showed significant reduction (p < 0.05) in liver enzyme activities, alongside increased total protein and globulin at 4.5% inclusion. Cholesterol decreased significantly (p < 0.05) in 3% and 4.5% inclusions, while triglycerides were lower in all BCSM groups compared to control. Antioxidant status significantly improved (p < 0.05), evidenced by increased catalase and glutathione peroxidase (GSH-Px) activities and a significant reduction in malondialdehyde (MDA) levels, particularly at 3% and 4.5%. In conclusion, BCSM serve as a multifunctional feed ingredient that can be included up to 4.5% to enhance meat quality, serum antioxidant capacity and key physiological functions in broilers.