Generation of a Brachyury reporter cell line (BJNhem20 Brachyury (TBXT)-2A-EGFP) in human embryonic stem cells using CRISPR-Cas9 gene targeting

Stem Cell Research 2026 Open access

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Authors: Shekhar Patil, Arghakusum Das, Maneesha S. Inamdar
DOI: 10.1016/j.scr.2026.103907
Cited by: 1
References: 5

Abstract

Brachyury is a key transcription factor, which is required for mesoderm lineage development. Here, we have generated a Brachyury/TBXT-2A-EGFP knock-in Reporter line in the BJNhem20 human embryonic stem cell line, using CRISPR/Cas9-based gene editing. Successful gene editing was verified by DNA sequencing and comparing endogenous gene expression to reporter gene expression. This reporter line represents an important tool for tracking and assessing mesoderm differentiation.

Fields of Study & Subjects

CRISPR and Genetic Engineering Pluripotent Stem Cells Research Single-cell and spatial transcriptomics

Publication details

Journal / source
Stem Cell Research
Publisher
Elsevier BV
Volume
91
Pages
103907–103907
Publication date
10 January 2026
Language
EN