The establishment of a GPD1L knockout human embryonic stem cell line (WAe009-A-80) using the CRISPR/Cas9 system

Stem Cell Research 2026 Open access

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Authors: Tao Dong, Yan Zhao, Haifeng Jin, H. Pan, Li-Ling Yue, Yan Lin, Lei Shen
DOI: 10.1016/j.scr.2026.103910
Cited by: 0
References: 3

Abstract

The GPD1L gene is located on 3p22.3. It encodes the glycerol phosphate dehydrogenase 1-like protein with homology to glycerol phosphate dehydrogenase (GPD1L), but the function of this enzyme is unclear. Mutations in GPD1L have been associated with BrS (Brugada syndrome) and SIDS (sudden infant death syndrome) and reduce Na + inward current through an unknown mechanism in human cardiomyocytes. Here, a GPD1L knockout human embryonic stem cell line was generated using CRISPR/Cas9 system. The GPD1L knockout human embryonic stem cell maintains the pluripotency, differentiation into three germ layers, forming normal EBs.

Fields of Study & Subjects

Pluripotent Stem Cells Research Congenital heart defects research Pancreatic function and diabetes

Publication details

Journal / source
Stem Cell Research
Volume
91
Pages
103910–103910
Publication date
9 January 2026
Language
EN