Generation of a homozygous TET2 knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential (CHIP)
Abstract
Atherosclerotic cardiovascular diseases (CVD) have been linked to mutations related to clonal hematopoiesis of indeterminate potential (CHIP), with Tet methylcytosine dioxygenase 2 (TET2) being the second most often altered gene. (Jaiswal et al. 2017; Abplanalp et al. 2021) We produced a genome-edited human induced pluripotent stem cell (hiPSC) line with a homozygous knock-out (KO) of TET2, simulating loss-of-function mutations, and a control line. We evaluated the pluripotency status of these lines and their capacity to differentiate into mesoderm, ectodem and endoderm. Analysis of the cellular pathomechanisms of TET2-related, CHIP-associated CVDs is made possible by the generation of these cell lines.
Fields of Study & Subjects
Acute Myeloid Leukemia Research
Zebrafish Biomedical Research Applications
Cardiomyopathy and Myosin Studies
Publication details
- Journal / source
- Stem Cell Research
- Publisher
- Elsevier BV
- Volume
- 92
- Pages
- 103942–103942
- Publication date
- 22 February 2026
- Language
- EN